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ccl4 obeticholic acid  (MedChemExpress)


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    MedChemExpress ccl4 obeticholic acid
    A Schematic diagram of the SPD treatment protocol. 8-week-old C57BL/6 J mice ( n = 6) received intraperitoneal injections of CCl₄ for 8 consecutive weeks to induce liver fibrosis. From the second week onward, SPD (3 mM) was added to the drinking water, and obeticholic acid (OCA, 5 mg/kg) was administered daily via oral gavage for the remaining 7 weeks. B Representative images of H&E and Sirius Red staining, as well as immunohistochemical staining for α-SMA, CD34, and LYVE1 in liver sections (bar = 100 µm). C Analysis of protein levels of COL1A1, α-SMA, CD34, and LYVE1 in liver tissues by western blot ( n = 3, performed in biological replicates). D , E Analysis of mRNA levels of COL1A1, α-SMA, TIMP-1, TNF-α, IL-1, LYVE1, STAB1, STAB2, CD209B, EHD3, CD34, EDN1, LAMB1 and PECAM1 in liver tissues by RT-qPCR ( n = 6, performed in biological replicates). F Representative TEM and SEM images of liver tissues from CCl₄ and CCl₄ +SPD group. G Schematic of the co-culture model. LX-2 cells were co-cultured with SPD-treated (or untreated) HUVEC. H Analysis of protein levels of CD34 and LYVE1 in HUVEC. I Analysis of protein levels of COL1A1 and α-SMA expression in co-cultured LX-2 cells. J Matrigel tube-formation assay in HUVEC ( n = 3, performed in biological replicates). Cells were pretreated with SPD (20 μM) for 24 h, then seeded on matrigel-coated 48-well plates and stimulated with VEGFA (25 ng/mL) for 24 h. Images were captured 24 h after seeding. Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.
    Ccl4 Obeticholic Acid, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl4+obeticholic+acid/Obeticholic+acid/pmc13320189-295-29-34
    Average 95 stars, based on 85 article reviews
    ccl4 obeticholic acid - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Spermidine suppresses liver fibrosis by remodeling the communication signal between liver sinusoidal endothelial cells and hepatic stellate cells"

    Article Title: Spermidine suppresses liver fibrosis by remodeling the communication signal between liver sinusoidal endothelial cells and hepatic stellate cells

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-026-03129-4

    A Schematic diagram of the SPD treatment protocol. 8-week-old C57BL/6 J mice ( n = 6) received intraperitoneal injections of CCl₄ for 8 consecutive weeks to induce liver fibrosis. From the second week onward, SPD (3 mM) was added to the drinking water, and obeticholic acid (OCA, 5 mg/kg) was administered daily via oral gavage for the remaining 7 weeks. B Representative images of H&E and Sirius Red staining, as well as immunohistochemical staining for α-SMA, CD34, and LYVE1 in liver sections (bar = 100 µm). C Analysis of protein levels of COL1A1, α-SMA, CD34, and LYVE1 in liver tissues by western blot ( n = 3, performed in biological replicates). D , E Analysis of mRNA levels of COL1A1, α-SMA, TIMP-1, TNF-α, IL-1, LYVE1, STAB1, STAB2, CD209B, EHD3, CD34, EDN1, LAMB1 and PECAM1 in liver tissues by RT-qPCR ( n = 6, performed in biological replicates). F Representative TEM and SEM images of liver tissues from CCl₄ and CCl₄ +SPD group. G Schematic of the co-culture model. LX-2 cells were co-cultured with SPD-treated (or untreated) HUVEC. H Analysis of protein levels of CD34 and LYVE1 in HUVEC. I Analysis of protein levels of COL1A1 and α-SMA expression in co-cultured LX-2 cells. J Matrigel tube-formation assay in HUVEC ( n = 3, performed in biological replicates). Cells were pretreated with SPD (20 μM) for 24 h, then seeded on matrigel-coated 48-well plates and stimulated with VEGFA (25 ng/mL) for 24 h. Images were captured 24 h after seeding. Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: A Schematic diagram of the SPD treatment protocol. 8-week-old C57BL/6 J mice ( n = 6) received intraperitoneal injections of CCl₄ for 8 consecutive weeks to induce liver fibrosis. From the second week onward, SPD (3 mM) was added to the drinking water, and obeticholic acid (OCA, 5 mg/kg) was administered daily via oral gavage for the remaining 7 weeks. B Representative images of H&E and Sirius Red staining, as well as immunohistochemical staining for α-SMA, CD34, and LYVE1 in liver sections (bar = 100 µm). C Analysis of protein levels of COL1A1, α-SMA, CD34, and LYVE1 in liver tissues by western blot ( n = 3, performed in biological replicates). D , E Analysis of mRNA levels of COL1A1, α-SMA, TIMP-1, TNF-α, IL-1, LYVE1, STAB1, STAB2, CD209B, EHD3, CD34, EDN1, LAMB1 and PECAM1 in liver tissues by RT-qPCR ( n = 6, performed in biological replicates). F Representative TEM and SEM images of liver tissues from CCl₄ and CCl₄ +SPD group. G Schematic of the co-culture model. LX-2 cells were co-cultured with SPD-treated (or untreated) HUVEC. H Analysis of protein levels of CD34 and LYVE1 in HUVEC. I Analysis of protein levels of COL1A1 and α-SMA expression in co-cultured LX-2 cells. J Matrigel tube-formation assay in HUVEC ( n = 3, performed in biological replicates). Cells were pretreated with SPD (20 μM) for 24 h, then seeded on matrigel-coated 48-well plates and stimulated with VEGFA (25 ng/mL) for 24 h. Images were captured 24 h after seeding. Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Staining, Immunohistochemical staining, Western Blot, Quantitative RT-PCR, Co-Culture Assay, Cell Culture, Expressing, Tube Formation Assay

    A Schematic Diagram of mice treatment. Briefly, mice ( n = 6) received intraperitoneal injections of CCl₄ and were administered with AAV vectors (AAV-control or AAV- Bgn ) via the tail vein at 10 weeks of age, followed by an 8-week SPD treatment. B Body weight, liver weight, liver/body weight ratio, serum ALT and AST levels in each group. C Representative images of H&E staining of liver sections (bar = 100 μM). D – G Effects of AAV- Bgn on LSECs dysfunction. D Analysis of BGN ubiquitination, BGN, UBE2G2, ERK/p-ERK, p38/p-p38, CD34, LYVE1 expression in the liver tissues by western blot. The arrow indicates the IgG heavy chain band. E Analysis of mRNA levels of LSECs markers and capillary endothelial markers in liver tissues ( n = 6, performed in biological replicates). F Immunofluorescence staining of LYVE1 and CD34 (bar = 100 μM). G Representative TEM and SEM images of liver tissues. Effects of AAV- Bgn on HSCs activation. H Representative images of Sirius Red staining and immunohistochemical staining for COL1A1 and α-SMA in liver sections (bar = 100 μM). I Analysis of related protein levels in the livers tissues ( n = 3, performed in biological replicates). J Analysis of mRNA levels of fibrotic markers in liver tissues ( n = 6, performed in biological replicates). Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: A Schematic Diagram of mice treatment. Briefly, mice ( n = 6) received intraperitoneal injections of CCl₄ and were administered with AAV vectors (AAV-control or AAV- Bgn ) via the tail vein at 10 weeks of age, followed by an 8-week SPD treatment. B Body weight, liver weight, liver/body weight ratio, serum ALT and AST levels in each group. C Representative images of H&E staining of liver sections (bar = 100 μM). D – G Effects of AAV- Bgn on LSECs dysfunction. D Analysis of BGN ubiquitination, BGN, UBE2G2, ERK/p-ERK, p38/p-p38, CD34, LYVE1 expression in the liver tissues by western blot. The arrow indicates the IgG heavy chain band. E Analysis of mRNA levels of LSECs markers and capillary endothelial markers in liver tissues ( n = 6, performed in biological replicates). F Immunofluorescence staining of LYVE1 and CD34 (bar = 100 μM). G Representative TEM and SEM images of liver tissues. Effects of AAV- Bgn on HSCs activation. H Representative images of Sirius Red staining and immunohistochemical staining for COL1A1 and α-SMA in liver sections (bar = 100 μM). I Analysis of related protein levels in the livers tissues ( n = 3, performed in biological replicates). J Analysis of mRNA levels of fibrotic markers in liver tissues ( n = 6, performed in biological replicates). Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Control, Staining, Ubiquitin Proteomics, Expressing, Western Blot, Immunofluorescence, Activation Assay, Immunohistochemical staining

    Related Articles

    Control:

    Article Title: Spermidine suppresses liver fibrosis by remodeling the communication signal between liver sinusoidal endothelial cells and hepatic stellate cells.
    Article Snippet: .. AR TI CL E IN P RE SS After a 2-week acclimatization period, the mice were randomly assigned to four groups (n = 6 per group): Control (Con), CCl4, CCl4 + Spermidine (SPD, Sigma-Aldrich, S0266), and CCl4 + Obeticholic Acid (OCA; MedChemExpress, HY-12222). ..

    Article Title: Spermidine suppresses liver fibrosis by remodeling the communication signal between liver sinusoidal endothelial cells and hepatic stellate cells
    Article Snippet: .. After a 2-week acclimatization period, the mice were randomly assigned to four groups ( n = 6 per group): Control (Con), CCl4, CCl4 + Spermidine (SPD, Sigma-Aldrich, S0266), and CCl4 + Obeticholic Acid (OCA; MedChemExpress, HY-12222). ..



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    ccl4 obeticholic acid  (MedChemExpress)
    95
    MedChemExpress ccl4 obeticholic acid
    A Schematic diagram of the SPD treatment protocol. 8-week-old C57BL/6 J mice ( n = 6) received intraperitoneal injections of CCl₄ for 8 consecutive weeks to induce liver fibrosis. From the second week onward, SPD (3 mM) was added to the drinking water, and obeticholic acid (OCA, 5 mg/kg) was administered daily via oral gavage for the remaining 7 weeks. B Representative images of H&E and Sirius Red staining, as well as immunohistochemical staining for α-SMA, CD34, and LYVE1 in liver sections (bar = 100 µm). C Analysis of protein levels of COL1A1, α-SMA, CD34, and LYVE1 in liver tissues by western blot ( n = 3, performed in biological replicates). D , E Analysis of mRNA levels of COL1A1, α-SMA, TIMP-1, TNF-α, IL-1, LYVE1, STAB1, STAB2, CD209B, EHD3, CD34, EDN1, LAMB1 and PECAM1 in liver tissues by RT-qPCR ( n = 6, performed in biological replicates). F Representative TEM and SEM images of liver tissues from CCl₄ and CCl₄ +SPD group. G Schematic of the co-culture model. LX-2 cells were co-cultured with SPD-treated (or untreated) HUVEC. H Analysis of protein levels of CD34 and LYVE1 in HUVEC. I Analysis of protein levels of COL1A1 and α-SMA expression in co-cultured LX-2 cells. J Matrigel tube-formation assay in HUVEC ( n = 3, performed in biological replicates). Cells were pretreated with SPD (20 μM) for 24 h, then seeded on matrigel-coated 48-well plates and stimulated with VEGFA (25 ng/mL) for 24 h. Images were captured 24 h after seeding. Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.
    Ccl4 Obeticholic Acid, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl4+obeticholic+acid/Obeticholic+acid/pmc13320189-295-29-34
    Average 95 stars, based on 1 article reviews
    ccl4 obeticholic acid - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    ccl4 obeticholic  (MedChemExpress)
    95
    MedChemExpress ccl4 obeticholic
    A Schematic diagram of the SPD treatment protocol. 8-week-old C57BL/6 J mice ( n = 6) received intraperitoneal injections of CCl₄ for 8 consecutive weeks to induce liver fibrosis. From the second week onward, SPD (3 mM) was added to the drinking water, and obeticholic acid (OCA, 5 mg/kg) was administered daily via oral gavage for the remaining 7 weeks. B Representative images of H&E and Sirius Red staining, as well as immunohistochemical staining for α-SMA, CD34, and LYVE1 in liver sections (bar = 100 µm). C Analysis of protein levels of COL1A1, α-SMA, CD34, and LYVE1 in liver tissues by western blot ( n = 3, performed in biological replicates). D , E Analysis of mRNA levels of COL1A1, α-SMA, TIMP-1, TNF-α, IL-1, LYVE1, STAB1, STAB2, CD209B, EHD3, CD34, EDN1, LAMB1 and PECAM1 in liver tissues by RT-qPCR ( n = 6, performed in biological replicates). F Representative TEM and SEM images of liver tissues from CCl₄ and CCl₄ +SPD group. G Schematic of the co-culture model. LX-2 cells were co-cultured with SPD-treated (or untreated) HUVEC. H Analysis of protein levels of CD34 and LYVE1 in HUVEC. I Analysis of protein levels of COL1A1 and α-SMA expression in co-cultured LX-2 cells. J Matrigel tube-formation assay in HUVEC ( n = 3, performed in biological replicates). Cells were pretreated with SPD (20 μM) for 24 h, then seeded on matrigel-coated 48-well plates and stimulated with VEGFA (25 ng/mL) for 24 h. Images were captured 24 h after seeding. Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.
    Ccl4 Obeticholic, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl4+obeticholic+acid/Obeticholic+acid/pm40912645-67-48-54
    Average 95 stars, based on 1 article reviews
    ccl4 obeticholic - by Bioz Stars, 2026-10
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    A Schematic diagram of the SPD treatment protocol. 8-week-old C57BL/6 J mice ( n = 6) received intraperitoneal injections of CCl₄ for 8 consecutive weeks to induce liver fibrosis. From the second week onward, SPD (3 mM) was added to the drinking water, and obeticholic acid (OCA, 5 mg/kg) was administered daily via oral gavage for the remaining 7 weeks. B Representative images of H&E and Sirius Red staining, as well as immunohistochemical staining for α-SMA, CD34, and LYVE1 in liver sections (bar = 100 µm). C Analysis of protein levels of COL1A1, α-SMA, CD34, and LYVE1 in liver tissues by western blot ( n = 3, performed in biological replicates). D , E Analysis of mRNA levels of COL1A1, α-SMA, TIMP-1, TNF-α, IL-1, LYVE1, STAB1, STAB2, CD209B, EHD3, CD34, EDN1, LAMB1 and PECAM1 in liver tissues by RT-qPCR ( n = 6, performed in biological replicates). F Representative TEM and SEM images of liver tissues from CCl₄ and CCl₄ +SPD group. G Schematic of the co-culture model. LX-2 cells were co-cultured with SPD-treated (or untreated) HUVEC. H Analysis of protein levels of CD34 and LYVE1 in HUVEC. I Analysis of protein levels of COL1A1 and α-SMA expression in co-cultured LX-2 cells. J Matrigel tube-formation assay in HUVEC ( n = 3, performed in biological replicates). Cells were pretreated with SPD (20 μM) for 24 h, then seeded on matrigel-coated 48-well plates and stimulated with VEGFA (25 ng/mL) for 24 h. Images were captured 24 h after seeding. Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Cell Death Discovery

    Article Title: Spermidine suppresses liver fibrosis by remodeling the communication signal between liver sinusoidal endothelial cells and hepatic stellate cells

    doi: 10.1038/s41420-026-03129-4

    Figure Lengend Snippet: A Schematic diagram of the SPD treatment protocol. 8-week-old C57BL/6 J mice ( n = 6) received intraperitoneal injections of CCl₄ for 8 consecutive weeks to induce liver fibrosis. From the second week onward, SPD (3 mM) was added to the drinking water, and obeticholic acid (OCA, 5 mg/kg) was administered daily via oral gavage for the remaining 7 weeks. B Representative images of H&E and Sirius Red staining, as well as immunohistochemical staining for α-SMA, CD34, and LYVE1 in liver sections (bar = 100 µm). C Analysis of protein levels of COL1A1, α-SMA, CD34, and LYVE1 in liver tissues by western blot ( n = 3, performed in biological replicates). D , E Analysis of mRNA levels of COL1A1, α-SMA, TIMP-1, TNF-α, IL-1, LYVE1, STAB1, STAB2, CD209B, EHD3, CD34, EDN1, LAMB1 and PECAM1 in liver tissues by RT-qPCR ( n = 6, performed in biological replicates). F Representative TEM and SEM images of liver tissues from CCl₄ and CCl₄ +SPD group. G Schematic of the co-culture model. LX-2 cells were co-cultured with SPD-treated (or untreated) HUVEC. H Analysis of protein levels of CD34 and LYVE1 in HUVEC. I Analysis of protein levels of COL1A1 and α-SMA expression in co-cultured LX-2 cells. J Matrigel tube-formation assay in HUVEC ( n = 3, performed in biological replicates). Cells were pretreated with SPD (20 μM) for 24 h, then seeded on matrigel-coated 48-well plates and stimulated with VEGFA (25 ng/mL) for 24 h. Images were captured 24 h after seeding. Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: After a 2-week acclimatization period, the mice were randomly assigned to four groups ( n = 6 per group): Control (Con), CCl4, CCl4 + Spermidine (SPD, Sigma-Aldrich, S0266), and CCl4 + Obeticholic Acid (OCA; MedChemExpress, HY-12222).

    Techniques: Staining, Immunohistochemical staining, Western Blot, Quantitative RT-PCR, Co-Culture Assay, Cell Culture, Expressing, Tube Formation Assay

    A Schematic Diagram of mice treatment. Briefly, mice ( n = 6) received intraperitoneal injections of CCl₄ and were administered with AAV vectors (AAV-control or AAV- Bgn ) via the tail vein at 10 weeks of age, followed by an 8-week SPD treatment. B Body weight, liver weight, liver/body weight ratio, serum ALT and AST levels in each group. C Representative images of H&E staining of liver sections (bar = 100 μM). D – G Effects of AAV- Bgn on LSECs dysfunction. D Analysis of BGN ubiquitination, BGN, UBE2G2, ERK/p-ERK, p38/p-p38, CD34, LYVE1 expression in the liver tissues by western blot. The arrow indicates the IgG heavy chain band. E Analysis of mRNA levels of LSECs markers and capillary endothelial markers in liver tissues ( n = 6, performed in biological replicates). F Immunofluorescence staining of LYVE1 and CD34 (bar = 100 μM). G Representative TEM and SEM images of liver tissues. Effects of AAV- Bgn on HSCs activation. H Representative images of Sirius Red staining and immunohistochemical staining for COL1A1 and α-SMA in liver sections (bar = 100 μM). I Analysis of related protein levels in the livers tissues ( n = 3, performed in biological replicates). J Analysis of mRNA levels of fibrotic markers in liver tissues ( n = 6, performed in biological replicates). Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Cell Death Discovery

    Article Title: Spermidine suppresses liver fibrosis by remodeling the communication signal between liver sinusoidal endothelial cells and hepatic stellate cells

    doi: 10.1038/s41420-026-03129-4

    Figure Lengend Snippet: A Schematic Diagram of mice treatment. Briefly, mice ( n = 6) received intraperitoneal injections of CCl₄ and were administered with AAV vectors (AAV-control or AAV- Bgn ) via the tail vein at 10 weeks of age, followed by an 8-week SPD treatment. B Body weight, liver weight, liver/body weight ratio, serum ALT and AST levels in each group. C Representative images of H&E staining of liver sections (bar = 100 μM). D – G Effects of AAV- Bgn on LSECs dysfunction. D Analysis of BGN ubiquitination, BGN, UBE2G2, ERK/p-ERK, p38/p-p38, CD34, LYVE1 expression in the liver tissues by western blot. The arrow indicates the IgG heavy chain band. E Analysis of mRNA levels of LSECs markers and capillary endothelial markers in liver tissues ( n = 6, performed in biological replicates). F Immunofluorescence staining of LYVE1 and CD34 (bar = 100 μM). G Representative TEM and SEM images of liver tissues. Effects of AAV- Bgn on HSCs activation. H Representative images of Sirius Red staining and immunohistochemical staining for COL1A1 and α-SMA in liver sections (bar = 100 μM). I Analysis of related protein levels in the livers tissues ( n = 3, performed in biological replicates). J Analysis of mRNA levels of fibrotic markers in liver tissues ( n = 6, performed in biological replicates). Data are represented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: After a 2-week acclimatization period, the mice were randomly assigned to four groups ( n = 6 per group): Control (Con), CCl4, CCl4 + Spermidine (SPD, Sigma-Aldrich, S0266), and CCl4 + Obeticholic Acid (OCA; MedChemExpress, HY-12222).

    Techniques: Control, Staining, Ubiquitin Proteomics, Expressing, Western Blot, Immunofluorescence, Activation Assay, Immunohistochemical staining